Disruption of BAF complex assembly affected the coregulation of YAP1 and β-catenin, indicating that this chromatin remodeling complex plays a crucial role for interdependent YAP1 and β-catenin activation in synovial sarcoma cells. Coimmunoprecipitation experiments detected nuclear interactions between YAP1, β-catenin, and the SS18-SSX fusion protein, the latter being an integral part of the BAF complex. These analyses showed the highest cooperative effect with overexpression of YAP1 in combination with β-catenin. If only one catalog number is used for the chemical, the full name and catalog number are linked to the safety data sheet. In vitro, small-molecule inhibitor treatment, RNAi-mediated knockdown, and vector-based overexpression assays demonstrated that YAP1, TAZ, and β-catenin transcriptional activity is not only stimulated by the SS18-SSX fusion protein, but that they also mutually enhance each other's activation. In a large cohort of synovial sarcoma tissue specimens, IHC analyses revealed a substantial subset of synovial sarcoma with concurrent nuclear accumulation of YAP1/TAZ and β-catenin. Search for safety data sheets (SDS) and certificates (COA/COO) by product number. Because previous functional analyses revealed a connection between SS18-SSX and the activity of the transcriptional coregulators YAP1/TAZ and β-catenin, respectively, this study examined a potential interdependence between these essential effector proteins in synovial sarcoma. An easy way to quickly purchase products used often. The Safety Data Sheets provided below are for chemical/products of the Sigma-Aldrich brand (Aldrich, Sigma, Sigma-Aldrich, Fluka and Supelco) that are currently in stock or were previously in stock within the School of Natural Sciences & Mathematics. The resulting chimeric SS18-SSX fusion protein drives synovial sarcoma pathogenesis by integrating into the BAF complex and dysregulating gene transcription. Synovial sarcoma, a rare malignant soft tissue tumor, is characterized by a specific chromosomal translocation t(X 18). Brij® 35 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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